Spectroscopic studies on the binding of a new quinolone antibacterial agent: sinafloxacin to DNA.
نویسندگان
چکیده
The binding of sinafloxacin to DNA has been studied by fluorescence spectroscopy and UV-vis absorption spectroscopy. The variations in the spectroscopic characteristics of sinafloxacin upon addition of DNA were observed. Salt concentration effect, KI quenching, fluorescence polarization, DNA denaturation experiments, melting temperature (T(m)) curves and viscosity measurements were studied to investigate the binding mechanism. It was proved that the fluorescence quenching of sinafloxacin is a result of the formation of sinafloxacin-DNA complex. Quenching constants were determined using the Stern-Volmer equation. From the experiment evidence, the major binding mode of sinafloxacin with DNA was evaluated to be the groove binding.
منابع مشابه
Spectroscopic evidence of Cu(II) and Zn(II) complexes having amino acid based Schiff base: A special emphasis on in vitro antimicrobial, DNA binding and cleavage studies
A new Schiff base ligand (L) obtained by the condensation reaction of N-acetylaceto-otoluidineand 2-aminopropanoic acid (an amino acid), is used to synthesize four mononuclearcomplexes of [MLCl] and [ML2] types (where M = Cu(II) and Zn(II); L = Schiff base) bykeeping the metal and ligand ratio as 1:1 and 1:2 respectively. This ligand and its complexeshave been characterized on the basis of diff...
متن کاملEffects of Vitamin K3 on Cytotoxicity and DNA-binding Properties of a Palladium(II) Complex as a Potential Antitumor Agent
In many diseases such as cancer, simultaneous use of two or more pharmacologically active agents will be more effective and have fewer side effects. In this study a new palladium(II) complex with formula of [Pd(phen)(py-dtc)]NO3 (where is phen 1,10-phenanthroline and py-dtc is n-propyldithiocarbamate), was synthesized. The cytotoxic activity of this complex was tested against leukemia K562 cell...
متن کاملEvaluation of antibacterial activity of new N-piperazinyl quinolone derivatives with 1,3,4.thiadiazole group
quinolones are broad-spectrum antibacterial agents.they have many clinical uses which are increasing.quinolones exert antibacterial activity primarily by inhibiting bacterial DNA gyrase.the inhibition of DNA gyrase by the quinolones are greatly influenced by the nature of the C-7 substituent on the quinolones molecule.substitution of bulky functional groupd is also possible in C-7 position.furt...
متن کاملSpectroscopic studies on the interaction of Fe3O4@CaAl LDH@Lamivudine with the calf thymus DNA
In this study, we were synthesized Fe3O4@LDH@Lamivudine and characterized by FT-IR spectroscopy,XRD and TEM. The interaction of this nanoparticle with CT-DNA was investigated by viscosity, circulardichroism (CD), Uv-Visible and fluorescence spectroscopy. Among all nanocarriers which applied as drugdelivery vectors, layered double hydroxides (LDHs) with exchangeable anions in t...
متن کاملDNA groove binding of an asymmetric cationic porphyrin and its Cu(II) complex: Resolved by spectroscopic, viscometric and molecular docking studies
In the present study, the interaction between water-soluble cationic asymmetric porphyrin, 5-(1-Hexadecyl pyridinium-4-yl)-10, 15, 20-tris (1-Butyl pyridinium-4-yl) Porphyrin Chloride, and its copper (II) derivative with calf thymus DNA (CT-DNA) were studied by means of spectroscopic techniques, viscosity measurements and molecular docking. The monitoring of the changes in visible absorbance sp...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
دوره 25 11 شماره
صفحات -
تاریخ انتشار 2009